The Technology

One editing toolbox under all three pillars.

Site-specific knock-in at a locus we choose, characterized in primary human cells — the shared engineering foundation beneath the banked donor products, the autologous tolerance work, and ECEP.

What the toolbox serves

One engineering foundation, three pillars.

The same editing approach is tuned differently in each pillar — donor cells engineered for acceptance, a patient's own cells engineered for tolerance, and a manufacturing concept to deliver either at the bedside.

01

Off-the-shelf

Donor HSPCs tuned along two axes — how the cells stay invisible, and how long the graft should last.

Pillar 01 →
02

Autologous

The patient's own HSCs engineered to educate their immune system — tolerance rather than invisibility.

Pillar 02 →
03

ECEP

A closed, automated concept for running the same engineering at the point of care.

Pillar 03 →
Editing precision

Site-specific knock-in, not semi-random integration.

Homology-directed repair puts the cassette at a locus we choose — with known copy number and endogenous regulation intact.

Approved lentiviral products integrate semi-randomly: variable copy number and expression, and an insertional-mutagenesis risk that has to be monitored for years.

Splitting the nuclease and the repair template across two vectors breaks the single-vector packaging limit, so a full cassette can be delivered.

Loci in the toolbox
B2M HLA-E CIITA iCasp9

Immune compatibility and safety are tuned per indication by which of these are engineered, and how. Construct-level design and preclinical data are available under CDA / NDA.

High-level schematic of Hemavive's two-vector homology-directed knock-in approach.
Two-vector homology-directed knock-in.
Preclinical evidence

Measured, not assumed.

Each editing configuration is characterized for on-target integration, expression and immune phenotype before it enters a program. All programs are preclinical.

Manufacturing is the next constraint.

ECEP is Hemavive's closed, automated concept for bringing engineering closer to the patient.

About ECEP